Size-Exclusion Chromatography of Circulating Cardiac Troponin T

Interpretation of cardiac troponin T behaviour in size-exclusion chromatography.

BACKGROUND Knowledge about the presence of intact cardiac troponin T (cTnT) and/or its immunoreactive fragments is of great value for the interpretation of cTnT clearance from the circulation. Until now there has been a lot of controversy about cTnT fragmentation. To provide an answer to this controversy, we investigated fragmentation of cTnT with size-exclusion chromatography (SEC), and confir...

Circulating cardiac troponin T in myocardial contusion.

STUDY OBJECTIVE Myocardial contusion may induce life-threatening complications, but its diagnosis is difficult. Circulating cardiac troponin T is considered a highly sensitive and specific marker of myocardial cell injury. We investigate the value of cardiac troponin T measurement in the diagnosis of myocardial contusion. DESIGN Prospective study. SETTING Level 1 trauma center. METHODS We...

Size Exclusion Chromatography of Polymers

In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...

Measurement of Albuminuria with Size-exclusion Chromatography

Size exclusion chromatography does not require pores.

Separation of macromolecules on the basis of their molecular weight by size exclusion chromatography has long been considered to be caused by the geometry-dependent partition of macromolecules between a continuous phase and the porous interior of a gel or cross-linked bead. The volume of a pore accessible to a solute is limited by its relative dimensions, so larger molecules will have access to...